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KMID : 1007520100190041063
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Food Science and Biotechnology
2010 Volume.19 No. 4 p.1063 ~ p.1068
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Effect of Selected Phytochemicals on Cell Proliferation in A549 Lung Cancer Cells
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Yoon Hyung-Eun
Lee Chang-Yong
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Abstract
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Effects of quercetin 3-β-D-glucoside, resveratrol, and curcumin on A549 lung cancer cell proliferation and the mechanism of these phytochemicals in regulating apoptosis and cell cycle arrest were investigated. A549 cells were treated with quercetin 3-β-D-glucoside, resveratrol, or curcumin at 37oC for 96 hr and cell viability was measured with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Proteins related to apoptosis and cell cycle in A549 cells were quantified with Western blotting assay. Quercetin 3-β-D-glucoside, resveratrol, and curcumin inhibited A549 cell proliferation in a dose-dependent manner (p<0.05). Quercetin 3-β-Dglucoside significantly decrease the expression level of CDK4 at concentrations of 5 mM and above (p<0.05). Curcumin lowered expression level of Bcl-2, CDK4, and cyclin D1 at concentrations of 100, 50, and above, and 50 mM and above, respectively (p<0.05). These results suggest that phytochemicals, which can be found in a normal diet, inhibit lung cancer cell proliferation and regulate the expression of the proteins involved in apoptosis and cell cycle.
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KEYWORD
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lung cancer, A549 cell, quercetin 3-β-Dglucoside, resveratrol, curcumin
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